Nutritious supplemental composition for suppression against onset of large intestinal cancer and manufacturing method thereof

ABSTRACT

In view of an acknowledgement that keeping a healthy body is a fundamental matter for overcoming cancer, it is an object of the present invention to provide composition containing well-balanced nutrition, having an effect to suppress the mutagenesis substances, having no sub-action even if the composition is continued to be taken as nutritious supplemental substance and capable of promoting healthy state. There are provided a nutritious supplemental composition for suppression against large intestinal cancer and its manufacturing method in which dietary fiber in a range of 15 wt % to 30 wt % in respect to a total amount of composition is contained in the dried koji fine powder including dead fungi of Aspergillus while keeping a capability of catalysis of groups of enzyme produced by Aspergillus.

BACKGROUND OF THE INVENTION

[0001] 1. Field of the Invention

[0002] This invention relates to a nutritious supplemental compositionfor suppression against onset of large intestinal cancer in which itshows a superior suppression against onset of large intestinal cancer.

[0003] In particular, in the present invention, rice bran is heated withsteam, Aspergillus, Aspergillus oryzae strain, for example, is mixedwith the rice bran, they are cultivated and ripened to make rice brankoji, Aspergillus is annihilated, and the enzyme groups produced byAspergillus are changed into the dried koji fine powder of dried powderunder a state in which a proper capability of catalysis of enzyme itselfis not lost.

[0004] Either dietary fiber aiming at removal of mutagen or plantprotein acting as nutritious element is contained in it and the presentinvention relates to the nutritious supplemental composition forsuppression against onset of large intestinal cancer and itsmanufacturing method.

[0005] 2. Description of the Related Art

[0006] Cancer is totally defined as malignant tumor in general. Ascauses for oncogenesis, they are roughly classified into chemicaloncogenesis with carcinogenic substance and viral oncogenesis.

[0007] As chemical oncogenesis substances, a tremendous number ofcarcinogen substances are applied. Also as to viral oncogenesis, it hasbeen apparent that the number of types exceed 100 types or more. Inaddition, it has become apparent that a reason why cancer occursconsists in the fact that a combination among the chemical oncogenesissubstances and genes owned by a specific person is related to it andmore than 100 type of oncogenes have been reported.

[0008] In recent years, aberrant crypt foci (ACF) appearing in a rat atan initial dosing time of carcinogenic substances (azoxymethane=AOM, forexample) has been noticed as precancerous lesion of large Intestinal,this lesion is acknowledged in its presence at a human, so that this isassumed to be one of the important indexes for prevention of onset oflarge intestinal cancer.

[0009] As mutagen changing a proper character of cell itself, there arepresent “direct mutagen” such as nitroso compound or antiseptic agent,for example, giving derangement directly to gene in the cell; and“indirect mutagen” activated in a human body by enzyme such asdecomposition product of amino acid or protein found in scorched meat orfish or like, for example.

[0010] It is said that aberrant crypt foci generated by the “mutagen” isprecancerous lesin of large intestinal and has a high possibility ofbecoming a large intestinal cancer in the future.

[0011] Experiment has shown it apparent that substance for suppressionagainst this “aberrant crypt foci” can suppress approximately by 70% thelarge intestinal cancer which is onset by the “aberrant crypt foci”.

[0012] Further, in opposition to the carcinostatic chemotherapy, aviewpoint has been set in recent years to a direct breakage of cancercell under application of function of biophylaxis of host through killercells or immunocomplement cell or enforcement of biological functionindirectly retracting cancer by reinforcing function of organism and aconcept of applying biological response modifier (BRM) has beenadvocated in a medical field.

[0013] Along with this trend, a study about medicines or foods foractivating an immune system has also been promoted and frequently shownthat the foods derived from bacteria or mycete, for example,lactobacillus lactis, lactic acid coccus, cultivated basidiomycetesmycelioid (Aspergillus), fruit body of mushroom or the like havestimulus activation against the surface of leukocyte membrane.

[0014] As a representing example of this material, both production anddischarging of cytokine (this is a general term of non-antibody proteinsuch as lymphokines assisting action of cellular inmmune by stimulatingeither monocyte or macrophage or the like with groups of solublesubstances discharged by sensitized limphocyte contacted with specificantigens. This may act as an intercellular regulation factor when animmune response occurs.) are applied through stimulation of immunitysystem with component (generally called as an LPS) constituted by ellwalls of β-glucan contained in plant or gram-negative bacteria such aslactic acid bacteria or the like.

[0015] In addition, it is often said that some foods derived from plantsor animals, for example, chlorella, Nemacystus decipiens and other seaweeds (sulfuric acid polysaccharide), green and yellow leaf vegetables(carotenoid and others), fruits or the like (polyphenols and the like),beans (soybean peptide), rice bran (arabinoxylan and others), nucleicacid (salmon DNA), crusts (chitin, chitosan), shells (zinc, selenium)and the like show a mechanism for adjusting metabolic activity inleukocyte cells. As its typical example, there is provided an activationof metabolism enzyme by sulfide contained in vegetables.

[0016] In turn, it has been made apparent academically that vegetables(spinach, field pea, burdock, yam, nettle tree mushroom, mushroom,ginkgo nut, corn, green peas, or the like) or fruits (orange, grapefruit, mandarin range, apple or the like) have superior actions andeffects against suppression against production of mutagen in a humanbody, inactivation of mutagen, suppression against metabolism activationof mutagen, inactivation against mutagen of metabolism activation andsuppression against fixation of mutation of gene or the like.

[0017] That is, a canceration cell shows a cytodieresis by transmittinga specific signal within the cell itself even if a certain signal aboutproliferation is not received due to irregular state in its receptorrelated to proliferation. In this case, it is known that pigment systemssuch as carotenoid or the like contained in vegetable juice and the likemay improve irregular state of receptor of cell.

[0018] As effective constituents acting as anti-mutagen substancescontained in these vegetables and the like, there are cited examplessuch as vitamins e.g. vitamin C, proline (a type of α-amino acid), thioproline, glutathione (having a reducing action), cysteine (α-amino acidcontaining sulfur atom, wherein —SH in its structure is important forbioactivity), compound of sulfur and hydrogen, and dietary fiber and thelike.

[0019] Reduction in carbohydrate metabolism function as derangement ofmetabolism function concerning nourishment has a substantial meaning asan index for aging, wherein a peak age in which carbohydrate metabolismfunction of a human is about 65-year age while a high-aged trend isprogressive, hypergasia in total function of nutritious metabolismincluding carbohydrate metabolism may product a lack in energy at eachof the secretion organs, resulting in that it becomes a major cause in abad condition in physical state to generate a series of syndrome.

[0020] As cancers were onset in Japanese people, tremendous number ofpeople suffering from gastric cancer were found overwhelmingly, althoughin recent years in Japan, a number of people taking meat foods has beenincreased and the number of some people taking vegetable foods has beendecreased to cause unbalanced foods to be eaten, resulting in that it isreported that a rate of onset of large intestinal cancer has beenincreased and country where people take much amount of fat shows a largenumber of mammary cancers. However, a rate of onset of mammary cancersin Japan also shows an increasing trend in number.

[0021] If it is assumed that a rate of death classified by statisticalage for cancers in recent Japan is defined as a numerical value of 1 forage 24 or 25, the rate of death over age 40 exceeds by 9 times at once.This value increases by leaps and bounds like 29 times in fifties, 70times in sixties and 152 times in seventies, respectively.

[0022] It is possible to say that this numerical value is proportionalto a trend of reduction in immunity function accompanied by a reductionin metabolism function caused by the aforesaid aging.

[0023] It is defined such that taking much amount of meat causes eitherClostridium perfringens or escherichiacoli colibacillus to becomepredominant within intestine and further promote proliferation oftoxbacteria, resulting in that blood derangement is produced byderangement of tissue or gangrene with gas derived from toxbacteria andlarge intestinal cancer becomes onset.

[0024] In addition, scorching of protein as found in meat or hamburgerunder application of heat causes carcinogen substances such as Trp-p-1,Trp-p-2 or the like to be generated, wherein the carcinogen substancesmay promote not only large intestinal cancer but also mammary cancer andprostatic cancer.

[0025] However, taking a large amount of vegetables or dietary fibersshows a serious question in view of meal configuration as well asdigestive capability,

SUMMARY OF THE INVENTION

[0026] The present inventor has performed a wider and deeper study todevelop a nutritious supplemental food that everybody can take in aneasy manner, showing a high food value and a superior absorbability inview of acknowledgement in study that suppression of aging of a humanbody is attained by intake of balanced nutrition and activation ofmetabolism through improved environment of tissue cell in organism,keeping a healthy condition itself results in activation of immunefunction to prevent and suppress various kinds of sicknesses.

[0027] As a result, dietary fiber is included in cereal constituentcontaining not devitalized enzyme with rice bran koji being applied asmajor material to generate constituent. This constituent has awell-balanced nutrition, contributes to improvement of environment ofcell to activate cell and further promote activation of a metabolismfunction and an immune function. Additionally, this constituent maysuppress active oxygen in a human body becoming one of mutagen andfurther suppress onset of aberrant crypt foci.

[0028] Further, the present inventor has searched the fact that6-phytase produced by Aspergillus in the constituent of the presentinvention decomposes phytin in a plant (calcium salt and magnesium saltof myoinosithexaphosphate), facilitates it to be absorbed, so that it isnot necessary to take a large amount of vegetables or dietary fiber, andthen the present inventor has invented this invention.

[0029] In the present invention, following technical means have been setin order to resolve the aforesaid problems.

[0030] That is, rice bran is steamed, Aspergillus strain colony iscultivated and ripened to attain rice bran koji, Aspergillus isdiminished to attain dried substances including dead fungi, enzymegroups produced by the Aspergillus strain colony are contained in thedried koji fine powder under a state in which catalysis of the enzymeitself is not lost. This constituent includes either dietary fiber orplant protein.

[0031] In this case, cereal or the like such as unpolished rice iscultivated by Aspergillus together with rice bran and lactic acidbacteria can be cultivated by Aspergillus.

[0032] A practical configuration of the present invention is as follows.

[0033] (1) A nutritious supplemental composition for suppression againstonset of large intestinal cancer, wherein a capability of catalysis ofenzyme groups produced by Aspergillus is held as it is and dietary fiberin a range of 15 wt % to 30 wt % in respect to a total amount ofcomposition is contained in dried koji fine powder including dead fungiof Aspergillus.

[0034] (2) A nutritious supplemental composition for suppression againstonset of large intestinal cancer, wherein a capability of catalysis ofenzyme groups produced by Aspergillus is held as it is and plant proteinin a range of 15 wt % to 30 wt % in respect to a total amount ofcomposition is contained in dried koji fine powder including dead fungiof Aspergillus.

[0035] (3) A nutritious supplemental composition for suppression againstonset of large intestinal cancer, wherein a capability of catalysis ofenzyme groups produced by Aspergillus is held as it is, dietary fiber ina range of 15 wt % to 30 wt % in respect to a total amount ofcomposition and plant protein in a range of 15 wt % to 30 wt % inrespect to a total amount of composition are contained in dried kojifine powder including dead fungi of Aspergillus.

[0036] (4) A nutritious supplemental composition for suppression againstonset of large intestinal cancer according to any one of (1) to (3),wherein dead fungi of lactic acid bacteria are also included in saiddried koji fine powder.

[0037] (5) A nutritious supplemental composition for suppression againstonset of large intestinal cancer according to any one of (1) to (4),wherein said dried koji is rice bran koji.

[0038] (6) A nutritious supplemental composition for suppression againstonset of large intestinal cancer according to (5), wherein either asingle or mixed crop koji selected from rice bran koji, barley koji,corn koji, bean koji, and barley bran koji are contained in said ricebran koji in a range of 5 wt % to 30 wt %.

[0039] (7) A method for manufacturing a nutritious supplementalcomposition for suppression against onset of large intestinal cancer,wherein after Aspergillus is cultivated and ripened at steamed ricebran, a temperature of ripened product is kept at 44° C. to 46° C., theripened product (koji) is formed into dried rice bran koji containingdead fungi of Aspergillus while keeping a capability of catalysis ofenzyme groups produced by Aspergillus and further changed into finepowder and dietary fiber powder is uniformly mixed with the dried ricebran koji fine powder under a state in which there is no difference intheir humidity.

[0040] (8) A method for manufacturing a nutritious supplementalcomposition for suppression against onset of large intestinal cancer,wherein after Aspergillus is cultivated and ripened at steamed ricebran, a temperature of ripened product is kept at 44° C. to 46° C., theripened product (koji) is formed into dried rice bran koji fine powdercontaining dead fungi of Aspergillus while keeping a capability ofcatalysis of enzyme groups produced by Aspergillus and further formedinto fine powder, and the plant protein powder is uniformly mixed withsaid dried rice bran koji powder under a state in which there is nodifference in their humidity.

[0041] (9) A method for manufacturing a nutritious supplementalcomposition for suppression against onset of large intestinal canceraccording to any one of (7), (8), wherein after Aspergillus and lacticacid bacteria are cultivated and ripened, the ripened product is formedinto dried rice bran koji containing dead fungi of Aspergillus andlactic acid bacteria while keeping a capability of catalysis of enzymegroups produced by Aspergillus.

[0042] Unpolished rice surface contains the rind, seed coat and powderlayers occupying by about 5% and embryo bud by about 3% of entireunpolished rice, and these portions peeled off by rice cleaningoperation are defined as rice bran. In addition to glycoprotein,minerals or vitamins, the embryo bud and seed coat of rice bran containphytin acid (myoinositheraphosphat, its abbreviated symbol IP₆) in theform of phytin (calcium salt, magnesium salt of phytin acid),myoinositol (this is known as a part in which phosphorus acid is removedfrom phytic acid, a part of vitamin B complex required for growth ofyeast or growth of mouse, and as carcinostatic substance), arabinoxylan,ferulic acid (having functions to diminish radical of anticoagulate,diminish active oxygen and prevent onset of large intestinal cancer),amino butyric acid (α-amino acid of which small amount is present inprotein, this is related to respiration of brain tissue),polysaccharide, antioxidant, and unsaturated fatty acid or the like.

[0043] Almost of natural proteins contained in embryo bud areglycoprotein coupled to sugar. Although a physiological meaning of sugarchain of glycoprotein is not yet explicated well, functions of sugarchain already known are deeply related to cognizance of inter-cells bydifference in shape of sugar chains, and migration in organism andproteinmetabolism of cell or the like, and it is considered thatinflammation caused by mutagenesis substances such as active oxygen orbacteria and the like may induce variation in sugar chain binding aswell as cognizance disability of inter-cell, resulting in that eitherimmunodeficiency or decompensation is produced.

[0044] Lactic acid bacteria decomposes sugar substances contained inrice bran. A certain academic report indicates that lactic acid bacteriadie out with heat of 41° C. or higher and the dead fungi have someeffects against constituents composing the cell that various kinds ofimmunologic reactions in organism are activated, and a high antitumoraction or infection protection is attained.

[0045] There is another academic report that a single dosage of lacticacid bacteria in addition to dietary fiber has an effect of scavenger(cleaning action) in which the number of cancer cells showing livermetastasis is restricted.

[0046] As the constituent in the present invention, dried powder such asplant leaves, stalks, seeds (beans, seeds) having much amount of fibroussubstances can be applied as dietary fibers.

[0047] As major dietary fibers constituted by protein, there areprovided cellulose acting as major constituents of a cell wall of plant(polysaccharide that is a basic substance of plant fiber occupying byabout ⅓ of the plant), hemicellulose (this is polysaccharide of plantcell wall closely related to xylan, manna, galactans and others), lignin(aromatic polymer substance present in ligneous cell wall), pectin(gelatin-like acid polysaccharide present as inter-cell substance ofplant), alginic acid (polysaccharide of phlegm forming a cell membraneof sea weeds) and glucomannan (polysaccharide having, as its majorconstituent, mannose) or the like.

[0048] It has been made apparent that polysaccharide (macromolecularsugar chain) activates leukocyte, stimulates a cytokine network, causeshormone system to act and further activates immune function.

[0049] These dietary fibers are classified as water-soluble dietaryfiber and insoluble dietary fiber. The water-soluble dietary fiber has afunction to reduce blood serum cholesterol and a function to suppressincreasing of blood sugar value in postprandial state.

[0050] Insoluble dietary fiber has a function to adsorb eithercarcinogen substances or carcinogen promoting substances in intestineand discharge them out of a human body. It is also reported that pectinof water-soluble dietary fiber has an effect to suppress largeintestinal cancer. In addition, a large amount of phytin acid andmyoinositol contained in a plant as a form of phytin are known ascarcinostatic substance.

[0051] It is acknowledged by an animal model induced with largeintestinal cancer that all phytic acid, myoinositol and inositolcompound are superior in prevention of onset of abberant crypt foci andan experiment with a nude mouse dosed with these combined substances hasreported that the mouse shows an effect of suppression of onsets oflarge intestinal cancer, fibrosarcoma and mammary cancer or the like.

[0052] For a human body, dosing of 1 to 2 g per day of phytic acid andmyoinositol shows an effect of prevention of large intestinal cancer andfor a person having a high risk of onset of large intestinal cancer, atwice amount of this volume is sufficient for prevention of it.

[0053] An academic report has shown that this phytic acid is not only acarcinostatic substance but also has prevention of arteriolosclerosis,prevention of senescence and anti-oxidation action.

[0054] In accordance with this academic report, an organism has enzymecalled as glutathione-S-transferase (GST) having a function todetoxicate chemical substances, i.e. mutagen coming from an externalenvironment into a human body and inactivate it, wherein when theexperiment rat is dosed with phytic acid (2% of feed), GST activationcharacteristic for inactivating chemical substances containing thesecarcinogen substances in a liver of rat increases with a significantdifference to cause mutagen substances to be decreased.

[0055] There is provided an academic report that phytic acid itself hasnot only an effect of prevention against large intestinal cancer, butalso has an effect that an effective effect of suppression against largeintestinal cancer under combination with green tea (concentration of 2%)can be expected, and an effect of suppression against large intestinalcancer can be improved more under combination with myoinositol havingphosphoric acid removed from phytic acid.

[0056] As for a rat, this phytic acid is decomposed by 6-phytase derivedfrom small intestine mucosa (enzyme processed with hydrolysis to remove6-phosphoric acid) and absorbed, although as for a human body,activation of 6-phytase derived from small intestine mucosa is quite lowas compared with that of rat, so that it can be scarcely absorbed unless6-phytase derived from meal is present even if this 6-phytase is taken.In addition, it is assumed that phytic acid is hardly absorbed under astate in which it is combined with protein.

[0057] In view of this meaning, protein decomposing enzyme in theconstituent of the present invention decomposes protein, 6-phytaseproduced by Aspergillus performs a hydrolysis operation against phytinand phytic acid to absorb phytic acid (IP₆), although it is decomposedinto IP₅, IP₄, IP₃ and myoinositol and the like, and it may becomeeasily absorbed. In addition, either magnesium or calcium or the like isseparated from phosphoric acid group and becomes easily absorbed.

[0058] With such an arrangement as above, even if a large amount ofdietary fibers containing phytin are not taken, it is possible to attaina substantial effect of phytic acid as well as a substantial effect ofmyoinositol. Phytic acid reaches various kinds of organ in an organismin about one hour after taking it and has an immediate effect realizingits action.

[0059] The constituent of the present invention containing much amountof these substances descried above is well balanced in its nutrition andcontains much amount of biophylaxis activating substances. When a persontakes about 20 g of this constituent every day as nutritioussupplemental food in a continuous manner, his or her regular foodtogether with this supplemental food under their synergism enablesenvironment of cells to be improved. As a result, energy is given to thecell, a metabolism function and immune function or the like can beimproved to enable keeping of a healthy condition to be carried out.

[0060] In addition, substances for suppressing “mutagen” abundantlycontained in this composition may eliminate “mutagen”, restrict onset ofprecancerous lesion of large intestinal cancer, resulting in that itrealizes a superior effect in suppression of large intestinal cancer.

[0061] In particular, when 6-phytase generated by Aspergillus is taken,it performs a hydrolysis work for phytic acid in a form of phytin tocause it to be absorbed well, so that it is possible to attain anefficient pharmacodynamic action of myoinositol as well as phytic acidcontained in the composition of the present invention even if a largeamount of vegetables or dietary fibers are not taken.

[0062] A “product temperature” herein defined is meant by a temperatureof raw material where heat is generated at a culture bed throughAspergillus cultivation. As “rice bran koji”, it also contains koji inwhich crop koji is mixed at a rate of 30% or less.

[0063] The present invention has the following superior effects.

[0064] (1) If a large amount of the composition of the present inventiondescribed in claim 1 are taken into a body, each of the enzymes of whichlarge amount are contained may perform a catalysis action within thebody. Protein is decomposed into low molecular moles to enable aminoacid to be supplied and then protein may act organically to composeamino acid for cells of organism. In addition, one part of the dietaryfiber is decomposed with enzyme, the other part of the dietary fiber isnon-soluble, goes to the colon, adsorbs carcinogen, discharges it out ofthe body, stimulates the intestine wall to eliminate constipation.

[0065] The dead fungi are decomposed with enzyme in the body, absorbedinto the body so as to activate immunologic reaction.

[0066] 6-phytase generated by Aspergillus in the composition of thepresent invention performs a hydrolysis action against phytic acid in aform of phytin to cause it to be easily absorbed.

[0067] Accordingly, even if a large amount of vegetables or dietaryfiber is not taken, either phytic acid or myoinositol can be efficientlyabsorbed, the composition may reach various kinds of organs in about onehour after a person takes this composition and it may attain anaccumulative effect capable of realizing its action in an immediateeffective manner.

[0068] Vitamins, minerals and other nutritious elements abundantlycontained in the composition of the present invention become energy ofcells in the organism and provide some effects that the environment ofcells is improved, metabolism function as well as immune functionproperly included in the cells are improved, these accumulative effectmay prevent aging to keep healthy condition of a human body and at thesame time the composition having action to suppress onset of largeintestinal cancer can be easily attained.

[0069] (2) The composition of the present invention described in claim 2contains plant protein in dried koji powder keeping a capability ofcatalysis of enzyme proper, so that in addition to the effects describedin the above item (1), it has some effects that even if a person doesnot supply protein additionally, mere taking of the present compositionenables the person to take sufficient plant protein, the protein issupplied to the cell as amino acid and acts against sugar chain of thecell and the composition of amino acid in an organic manner. With suchan arrangement as above, the present invention has some effects capableof contributing to recover a healthy condition and keep it as well andattaining composition for suppressing onset of large intestinal cancerthrough its accumulative action.

[0070] (3) The composition of the present invention described in claim 3contains dietary fibers and plant protein, so that it has an effectcombined with some effects described in the above items (1) and (2).

[0071] (4) The composition described in claim 4 is composition havingless amount of sugar by lactic acid bacteria and dead fungi of lacticacid bacteria shows some effects of attaining stimulation of immunesystem with the component of cell wall of lactic acid bacteria,production of cytokine and its discharging as well.

[0072] (5) The invention described in claim 5 has an effect thatnutrition at the embryo bud of unpolished rice can be utilizedconcentrically.

[0073] (6) The composition described in claim 6 contains either a singleor mixed crop koji selected from rice koji, barley koji, corn koji, beankoji, and barley bran koji, so that they may supplement each of poorsubstances in the food and provide an effect preferably accumulativeone.

[0074] (7) The manufacturing method described in claim 7 can manufactureuniform rice bran koji. In addition, dried rice bran koji fine powdercan be provided in such a way that the groups of enzyme produced byAspergillus may not lose a capability of catalysis of enzyme proper.When the dietary fiber fine powder is mixed with the rice bran koji finepowder, both of them are mixed to each other under a state in which nodifference occurs in temperature and humidity, thereby they can be mixedin a uniform density, resulting in that the mixed composition has aneffect that the composition superior for suppression against onset oflarge intestinal cancer can be easily manufactured.

[0075] (8) The manufacturing method described in claim 8 can manufactureuniform rice bran koji. In addition, dried rice bran koji fine powdercan be provided in such a way that the groups of enzyme produced byAspergillus may not lose a capability of catalysis of enzyme proper.When the plant protein fine powder is mixed with the rice bran koji finepowder, both of them can be mixed to each other under a state in whichno difference occurs in temperature and humidity, thereby they can bemixed in a uniform density, resulting in that the mixed composition hasan effect that the composition superior for suppression against onset oflarge intestinal cancer can be easily manufactured.

[0076] (9) The manufacturing method described in claim 9 has an effectthat dried rice bran koji containing less amount of sugar by decomposingsugar produced by lactic acid bacteria.

DESCRIPTION OF THE PREFERRED EMBODIMENTS

[0077] Preferred embodiments of the present invention will be describedas follows.

[0078] Method Preferred Embodiment 1

[0079] Selected rice bran is put into a water applying machine, water byabout 25 wt % to 30 wt % in regard to raw material is added, water issufficiently absorbed into the selected rice bran to cause this bran tobe bulged. The bulged bran is put into a steamer, calcareous powder(baked oyster shell powder=grain size #250 mesh to #400 mesh) is addedby about 0.5 wt % to 1 wt %, respectively, and these materials aresteamed with steam of 100° C. to 120° C. for about 50 to 60 minuteswhile their moisture being checked. When crops such as unpolished riceare applied, they are steamed together with rice bran.

[0080] Rice bran completely steamed up is released to cool down to about37° C. to 40° C. and Aspergillus strain colony, Aspergillus oryzaeccfstrain, for example, is added by about 0.1 wt % and they are wellmixed to each other.

[0081] In this case, it is possible to use Aspergillus selected fromother kojis (A. Kawachii, A. Awamori, A. Usami). In addition, as needed,a small amount of lactic acid bacteria (of about 2.5×10 pieces/g to3.0×102 pieces/g) is added. If required, the same amounts of yeast andlactic acid bacteria as those of Aspergillus can be added.

[0082] Aspergillus strain colony vigorously secretes decomposing enzymessuch as amylase, protease and lipase or the like to decompose sugar andprotein contained in rice bran into low molecules. The lactic acidbacteria decomposes sugar. Temperature suitable for cultivation ofAspergillus and lactic acid bacteria is 35° C. to 37° C. and humidity is85%RH to 90%RH, and activity in bacteria is stopped under a humidity of70%RH or less. Raw material having Aspergillus and lactic acid bacteriais cultivated for 32 to 36 hours while temperature and humidity arebeing adjusted in a stepwise manner in a range of room temperature of35° C. to 37° C. and in a range of humidity of 50%RH and 90%RH.

[0083] The rice bran at the culture bed are not closely contacted witheach other by calcareous powder uniformly adhered to the surfaces of therice bran to form a proper degree of aeration clearance and Aspergillusstrain colony is cultivated under a state where air flow is well kept inan average manner.

[0084] At the culture bed, its temperature is increased automaticallyfrom a temperature of 25° C. to 38° C. gradually under application ofcultivation heat of the Aspergillus strain colony. This state is keptfor a minimum time of 8 hours. When a temperature of product (atemperature of raw material in the cultivation bed) becomes 40° C. to41° C., the culture bed is agitated to cause the temperature of productto be forcedly decreased in such a way that an activity of speciesbacteria is not made dull. At this time, the temperature of product isdecreased down to about 32° C. and increased again up to a certaintemperature.

[0085] A growing period in which ripening of Aspergillus strain colonyis promoted is controlled at a room temperature of 31° C. to 36° C. insuch a way that the temperature of the product does not exceed 40° C.,and when the temperature of the product becomes 40° C. to 42° C. after 6hours, the culture bed is agitated to decrease the temperature of theproduct. After this operation, both temperature and humidity areadjusted in such a way that the temperature of the product may become arange of 39° C. to 40° C. and then the material is cultured for 11 hoursto 12 hours. Since the Aspergillus strain colony is aerobe, theagitation of culture bed is carried out for three to four times forevery specified time (8 hours) during this period to supply oxygen, acolor check and an odor check are performed, and further a ripening anda degree of dry are adjusted through agitation of the culture bed.

[0086] At a period of standstill in which growing of the Aspergillusstrain colony is stopped, the humidity is adjusted to 70%RH or lesswhere activity of Aspergillus is made dull and the room temperature isincreased up to 36° C. to 37° C. The temperature of the product isadjusted to a range of 39.5° C. to 40° C., the product is ripened for 6hours to attain rice bran koji. At this time, a value of pH at theculture bed is kept at 6 to 7.

[0087] After this operation, the rice bran koji at the culture bed isdried for 9 hours at a room temperature of 46° C. to 53° C. as it is.The temperature of the product is restricted in a range of 44° C. to 46°C., activities of Aspergillus and lactic acid bacteria are completelystopped and die out. Upon completion of drying operation, the productbecomes a dried material with a component moisture being less than 4%,2.5% to 3.6%, respectively. In this connection, the component moistureof normal rice bran is 13 to 14%.

[0088] This dried rice bran koji is applied to a screen to remove blockrigid product with a size of 5 mm or more, applied to a pulverizer toattain dried koji fine powder while a degree of fine powder beingchecked.

[0089] As applied unpolished rice, three kinds of rice of differentproducing districts were mixed to each other. As calcareous powder,baked oyster shell powder was selected. However, bone powder, calciumlactate, crab shells or the like can be applied and so the type of rawmaterial is not limited.

[0090] In the case of the aforesaid method, lactic acid bacteria addedin addition to Aspergillus is anaerobic, so that sugar is decomposed anddecreased in the layer where less amount of air flow is produced at theraw material in the culture bed. This lactic acid bacteria also dies outwith heat at the time of drying stage.

[0091] Method Preferred Embodiment 2

[0092] Either single crop or mixed crops selected from unpolished rice,barley, corn, soybean and barley bran are mixed with the rice bran in arange of 5 to 30 wt %, steamed and the cultivation with Aspergillus andlactic acid bacteria is carried out in the same manner as that of thepreferred embodiment 1. A drying step after ripening is also the same asthat of the preferred embodiment 1. These crops can be cultivated withAspergillus separate from rice bran and mixed with it.

[0093] Then, non-heated dried dietary fiber fine powder (moisturecontent of 2.5% to 3.6%) of 2 wt % to 30 wt % of a total amount ofcomposition is added to and mixed with the dried koji fine powdermanufactured in this way so that there occurs no difference in humiditybetween the dried koji fine powder and the non-heated dried dietaryfiber fine powder. That is, they are fine powder, so that if there is asubstantial difference in humidity between these materials, a certainblock may easily be formed when they are mixed to each other and atrouble may occur in a uniform mixing.

[0094] As the dietary fiber added to the dried koji fine powder, it ispossible to cite, as an example for it, fine powder of all driedmushrooms (shiitake, maitake, eringi, gano-derma lucidum and others),thumbmurray (G. pentaphyllum), kidney bean, field pea, ashida, mugwort,spinach, Brassica Rapa var. pervidis, tea leaf, burdock, bamboo shoot,carrot, onion, green pepper, konjak, sweet potato, ginger, seed ofvegetable (soy bean), rice bran, wheat bran powder, soy shell powder,sea weeds, fruits, persimmon leaf, bamboo leaf and other materials.Since these materials are powder of whole dried substances, otherseveral nutritious constituents in addition to fibers are abundantlycontained.

[0095] The dietary fibers of these vegetables and fruits show animportant relation for recovering metabolic disease, adsorb mutagenesissubstances in a large intestinal and eliminate them, so that theirphysiologic functions have been noticed. In addition, the dietary fibersstimulate the wall of intestine to promote hyper peristalsis andeliminate constipation.

[0096] As a drying step for vegetables and fruits, it is preferable toapply a non-heated frozen low temperature drying. The dietary fiber finepowder having vegetables dried can be mixed in a range of 2 wt % to 30wt % in respect to a total amount of constituent. Further, the dietaryfibers extracted from these vegetables can be applied. BlendingPreferred Embodiment 1 Dried rice bran koji fine powder 75 wt % Driedrice koji fine powder 10 wt % Soybean fine powder 10 wt % Thumbmurrayfine powder 3 wt % Mugwort fine powder 2 wt % Blending PreferredEmbodiment 2 Dried rice bran koji fine powder 80 wt % Dried crop kojifine powder 10 wt % Ashitaba leaf fine powder 4 wt % Spirulina finepowder 4 wt % Tea leaf fine powder 2 wt % Blending Preferred Embodiment3 Dried rice bran koji fine powder 70 wt % Dried rice koji fine powder10 wt % Mushroom (Ganoderma) fine powder 5 wt % Ashitaba leaf finepowder 7 wt % Tea leaf fine powder 2 wt % Ginger powder 1 wt % Soy beanprotein 5 wt % Blending Preferred Embodiment 4 Dried rice bran koji finepowder 80 wt % Ashitaba leaf fine powder 2 wt % Kidney bean fine powder2 wt % Seaweed fine powder 1 wt % Soy bean protein 15 wt % BlendingPreferred Embodiment 5 Dried rice bran koji fine powder 80 wt % Driedrice koji fine powder 10 wt % Seaweed (Hizikia fusiforme) fine powder 3wt % Spirulina 5 wt %

[0097] Mushrooms (Maitake, gano-derma lucidum, Agarisk, shiitake[Lentinus edodes] and others) contain much amount of constituentssuppressing mutagenesis substances, such as fibrous constituents, phyticacid in a form of phytin, β-glucan or the like. In particular, it hasalready been made clear that β-glucan breaks tumor cell by activatingimmunocomplement cell in organism, resulting in that retrocession ofcancer is attained. It is also reported that an experiment on prostaticcancer cell of a human body acknowledged that β-D-glucan induces aself-destruction inducing action.

[0098] Gingers contain protein decomposing enzyme and have ananti-bacteria action.

[0099] Seaweeds contain much amount of fibrous substances and also muchamount of iodine promoting activation of hormone of hypophysis cerebri.A requirement of iodine per day for an adult person is set to 300 to 400μg.

[0100] In addition, it is also possible to mix non-heated plant proteinof 5 wt % to 30 wt % in respect to a total amount of composition withdried koji fine powder.

[0101] As the plant protein, it is possible to cite soy bean powder, soybean protein, Spirulina and corn powder or the like. These substancessupply non-heated protein and decompose enzyme in a human body to absorbamino acid.

[0102] Spirulina described above is a blue-green algae well grown insalt water, contains fibrous substance and chlorophyll and 60% of itsentire volume is protein having stable amino acid. Blending PreferredEmbodiment 1 Dried rice bran koji fine powder 70 wt % Dried rice kojifine powder 10 wt % Soybean protein 20 wt % Blending PreferredEmbodiment 2 Dried rice bran koji fine powder 65 wt % to 80 wt % Soybeanprotein 20 wt % to 35 wt % Blending Preferred Embodiment 3 Dried ricebran koji fine powder 70 wt % Spirulina powder  5 wt % Soy bean protein25 wt % Blending Preferred Embodiment 4 Dried rice bran koji fine powder85 wt % Dried crop koji fine powder 10 wt % Spirulina powder  5 wt %

[0103] It is possible that dietary fiber fine powder with a range of 2wt % to 30 wt % in respect to a total amount of composition and plantprotein fine powder with a range of 5 to 30 wt % in respect to a totalamount of composition are mixed with dried koji fine powder. BlendingPreferred Embodiment 1 Dried rice bran koji fine powder 70 wt % Driedrice koji fine powder 10 wt % Soybean protein 15 wt % Seaweeds finepowder 2 wt % Ashitaba fine powder 3 wt % Blending Preferred Embodiment2 Dried rice bran koji fine powder 65 wt % Dried rice koji fine powder10 wt % Soy bean protein 20 wt % Konjak powder 1 wt % Barley bran finepowder 1 wt % Shiitake [Lentinus edodes] fine powder 3 wt % BlendingPreferred Embodiment 3 Dried rice bran koji fine powder 70 wt % Driedrice koji fine powder 5 wt % Soy bean protein 10 wt % Barley bran finepowder 1 wt % Konjak fine powder 3 wt % Ashitaba fine powder 3 wt %Shiitake [Lentinus edodes] fine powder 5 wt % Seaweeds fine powder 2 wt% Tea leaf fine powder 1 wt %

[0104] Excipient material to be described later is added to single driedkoji fine powder and mixed fine powder, they are formed into particleswith a grain size of 1.2 mm to 1.3 mm by a granulating machine, packagedinto a bag with weight of 3.5 g, 5 g and the like of one package by apackaging machine after drying operation, or not formed into granule,but into tablet by a tablet forming machine.

[0105] Although as the aforesaid excipient material, the raw material isnot limited to this, the raw materials to be described later areoptionally selected under a range of mixing, they are blended beforeforming particles (forming tablet), for example, to make particles orform tablets. Glucose  4 wt % to 12 wt % Lactose 12 wt % to 20 wt %Plant oil and fat 2 wt % to 6 wt %

[0106] A result of accumulative analysis of major compositions per 100 gof composition (rice bran koji by 65%, rice koji by 10%, soy beanprotein by 20% and shiitake [Lentinus edodes] powder by 5%) manufacturedin this way is as follows. Number of lactic acid bacteria 248,000pieces/g to 253,000 pieces/g Titer of amilase 3200 U/g to 3400 U/g Titerof neutric protease  366 U/g to 383 U/g Titer of lipase  524 U/g to 536U/g Saccharide 18.2 g to 18.5 g Protein (including amino acid) 22.4 g to24.8 g Dietary fiber 18.5 g to 19.3 g Phytic acid 3.86 to 4.23 gMoisture  2.6 g to 3.4 g Energy  426 to 445 Kcal

[0107] Since the dried vegetables contain fiber by 70% or more, it ispossible to increase an amount of inclusion of dietary fiber in thecomposition by 20% to 40% in reference to a blending amount. With suchan increased amount of fiber, an amount of phytic acid can also beincreased.

[0108] It is possible to increase an amount of inclusion of protein inthe composition of the present invention in proportion to an addedamount of the plant protein. Each of the aforesaid blending amounts isnot limited to the indicated values.

[0109] The present inventor performed a short term experiment underapplication of rats for checking prevention of oncogenesis andsuppression of onset with anti-mutagenesis substances, in particular,precancerous lesion of large intestinal cancer being applied as index ofthe composition of the present invention.

[0110] In the instant example, the present inventor performed studyabout prevention of oncogenesis of large intestinal cancer underapplication of composition of the present invention by targetazoxymethane=AOM acting as carcinogens with an aberrant crypt fociinduced at a rat large intestinal being applied as an index. As thecomposition of the present invention, substances of rice bran koji by65%, rice koji by 10%, soy bean protein by 20% and gano-derma lucidumfine powder by 5 wt % were used.

[0111] (1) Method of Experiment

[0112] Male rats F344 with 5-week age in five groups were used andazoxymethane by 15 mg/kg was dosed subcutaneously at each of 12 rats ofone group to four groups for one week and for two weeks after startingthe experiment.

[0113] As for second to fourth groups, the composition of the presentinvention was mixed with a fundamental meal at a concentration of eachof 1.25%, 2.5% and 5% and dosed in feed for each of experimental periods(five weeks).

[0114] As for the fifth group (four rats), no azoxymethane was applied,but only the composition of the present invention was mixed by 5% andfed to the rats.

[0115] Upon completion of the experiment, the rats were killed and theircolons were fixed in formalin, thereafter they were dyed with methyleneblue and a quantitative observation of aberrant crypt foci was carriedout.

[0116] (2) Result of Experiment

[0117] In each of the groups, no significance difference was found intheir average weight. Aberrant crypt foci of colon was acknowledged onlyat first to fourth groups. Each of average aberrant crypt foci for largeintestinals in the first to fourth groups was First group: 139.5 ± 27.7Second group: 122.6 ± 27.7 Third group:  99.0 ± 24.1 Fourth group:  79.0± 18.4

[0118] and each of the number of aberrant crypt foci of the third andfourth groups was significantly low (P<0.01) as compared with that ofthe first group.

[0119] In the case that they were classified in reference to the numberof foci constituting the aberrant crypt foci, the value was low for onefoci (P<0.05 or P<0.01) in any of the second to fourth groups ascompared with the first group, the value was low for two foci (P<0.05 orP<0.01) in any of the third and fourth groups as compared with the firstgroup, and the value was low (P<0.05) in more than four foci as comparedwith that of first group.

[0120] (3) Consideration

[0121] Study of this experiment has made it clear that dosing of thecomposition of the present invention may suppress onset of aberrantcrypt foci induced at a rat colon. It is estimated that this effect ofsuppression of onset of aberrant crypt foci causes the composition ofthe present invention to have the prevention of large intestinal cancerof a human body.

[0122] Next, the present inventor performed experiment for a long periodof time for the prevention of oncognesis under application of thecomposition of the present invention in the model of oncognesis of ratlarge intestinal cancer induced by carcinogens, i.e. azoxymethane=AOMfor the large intestinal cancer.

[0123] (1) Method of Experiment

[0124] Male rats F344 of five-week age were classified into 7 groupswith one group being constituted by 30 rats, sixth group and seventhgroup were excluded, and azoxymethane=AOM of 15 mg/kg was dosedsubcutaneously by a total of three times once in every week from thefirst week to the third week after starting the experiment.

[0125] First group was fed with basic food for an experimental period.Each of the second group and the third group was fed with mixedsubstances of 5% and 2.5% of the composition of the present invention,respectively, for five weeks after starting the experiment, andsubsequently, they were fed with the basic food.

[0126] The fourth group and the fifth group were fed with the basic foodup to the fifth week, each of 5% and 2.5% of the composition of thepresent invention was mixed with the basic food and fed.

[0127] The sixth group and the seventh group were not fed withazoxymethane=AOM and only 5% of the composition of the present inventionand the basic food were fed to them during the period of experiment.

[0128] After feeding for 40 weeks, all the rats were killed and a rateof onset and the number of onset of the tumor at the colon werecompared.

[0129] (2) Result of Experiment

[0130] At the time of killing the rats, an average weight in each of thegroups showed a slight decreasing trend in the group applied withazoxymethane as compared with that of non-processed group (the seventhgroup). Further, in the groups (the fourth, fifth and sixth groups)where the composition of the present invention has been dosed for a longperiod of time, a significant difference was not found in a weight ratioof liver and kidney of each of the groups.

[0131] Examination with the naked eye showed that the large intestinaltumor was found only in the first to fifth groups.

[0132] An incidence having a tumor in the first to fifth groups was;First group: 51.9% (14/27) Second group: 55.6% (15/27) Third group:47.8% (11/23) Fourth group: 18.5% (5/27) Fifth group: 34.6% (9/26) and

[0133] it was confirmed that a trend of suppression against onset oftumor in the fourth and fifth groups occurred in response toconcentration as compared with that of the first group, and the fourthgroup showed a decrease with a significant difference (P<0.05).

[0134] The number of onset of average tumor (multiplicity) per one ratin each of the groups was; First group: 0.77 ± 0.89 Second group: 0.70 ±0.91 Third group: 0.68 ± 0.83 Fourth group: 0.18 ± 0.39 Fifth group:0.42 ± 0.58 and

[0135] the fourth group showed a significant decrease as compared withthat of the first group (P<0.01).

[0136] (3) Consideration

[0137] In the case of model of oncogenesis of large intestinal cancer ina rat induced by azoxymethane, the composition of the present inventiondid not show the effect of suppression in the groups (the second andthird groups) fed with it at an initiation phase, although in the groups(the fourth and fifth groups) where the composition of the presentinvention was fed at a promotion phase, it showed an effect ofsuppression in response to a concentration and further an apparentsuppression effect was found in the group (the fourth group) fed with 5%of the composition of the present invention.

[0138] As described above, the composition of the present invention isassumed to be promising as the suppression substance against onset ofthe large intestinal cancer in a human body. An amount of composition ofthe present invention which is efficient after a person takes it isabout 14 g to 28 g per day and as the nutritious supplemental food takenfor the sake of keeping health, about 14 g of it is satisfactory. As astandard value, 21 g per day is preferable.

[0139] An amount of inclusion of phytic acid in the test substancedescribed above in this 21 g is 0.84 g to 0.93 g. Accordingly,increasing of an amount of addition of dietary fiber enables an amountof inclusion of phytic acid also to be increased.

[0140] To the contrary, as a result of performing the experiment for 29weeks by dosing bran containing much amount of inclusion of phytic acidand a single phytic acid of the same amount as that contained in thebran to the model of onset of oncogenesis of large intestinal cancerinduced by azoxymethane under the same condition, the group dosed withphytic acid was acknowledged to have a significant difference (a canceronset frequency of 52.6%), although the group dosed with bran was notacknowledged to have a significant difference (a cancer onset frequencyof 70%).

[0141] In view of this fact described above, it was acknowledged thateven if a large amount of plant containing much amount of phytic acidwas taken, an effect of suppression against onset of large intestinalcancer corresponding to an amount could not be expected.

[0142] An effect of herb medicine does not consist in an immediateeffect, but improve a disease while recovering a health of human body.The composition of the present invention also contains much amount ofnutritious elements as well as much amount of carsinostatic substances,and in particular, the fact that it contains decomposing enzyme forphytic acid provides a superior effect over suppression of onset againstlarge intestinal cancer, resulting in that it may provide nutrition forimproving environment of tissue cell and energy as well as improving ametabolism function and immune function and it has an effect acting as aherb medicine as well.

[0143] Further, the present invention is not limited to the aforesaidexample of form, but both selection of raw materials such as dietaryfiber and plant protein or the like and their blending rate can beoptionally set on a case-by-case base.

[0144] Although the present invention has been described with referenceto the preferred embodiments, it is apparent that the present inventionis not limited to the aforesaid preferred embodiments, but variousmodifications can be attained without departing from its scope.

1. A nutritious supplemental composition for suppression against onsetof large intestinal cancer characterized in that a capability ofcatalysis of enzyme groups produced by Aspergillus is held as it is anddietary fiber in a range of 15 wt % to 30 wt % in respect to a totalamount of said composition is contained in dried koji fine powderincluding dead fungi of Aspergillus.
 2. A nutritious supplementalcomposition for suppression against onset of large intestinal cancercharacterized in that a capability of catalysis of enzyme groupsproduced by Aspergillus is held as it is and plant protein in a range of15 wt % to 30 wt % in respect to a total amount of said composition iscontained in dried koji fine powder including dead fungi of Aspergillus.3. A nutritious supplemental composition for suppression against onsetof large intestinal cancer characterized in that a capability ofcatalysis of enzyme groups produced by Aspergillus is held as it is,dietary fiber in a range of 15 wt % to 30 wt % in respect to a totalamount of composition and plant protein in a range of 15 wt % to 30 wt %in respect to a total amount of said composition are contained in driedkoji fine powder including dead fungi of Aspergillus.
 4. A nutritioussupplemental composition for suppression agains onset of largeintestinal cancer according to any one of claims 1 to 3 characterized inthat dead fungi of lactic acid bacteria are also included in said driedkoji fine powder.
 5. A nutritious supplemental composition forsuppression against onset of large intestinal cancer according to anyone of claims 1 to 4 characterized in that said dried koji is rice brankoji.
 6. A nutritious supplemental composition for suppression againstonset of large intestinal cancer according to claim 5 characterized inthat either a single or mixed crop koji selected from rice bran koji,barley koji, corn koji, bean koji, and barley bran koji are contained insaid rice bran koji in a range of 5 wt %to 30 wt %.
 7. A method formanufacturing a nutritious supplemental composition for suppressionagainst onset of large intestinal cancer characterized in that afterAspergillus is cultivated and ripened at steamed rice bran, atemperature of ripened product is kept at 4420 C. to 46° C., the ripenedproduct (koji) is formed into dried rice bran koji containing dead fungiof Aspergillus while keeping a capability of catalysis of enzyme groupsproduced by Aspergillus and further changed into fine powder and dietaryfiber powder is uniformly mixed with the dried rice bran koji finepowder under a state in which there is no difference in their humidity.8. A method for manufacturing a nutritious supplemental composition forsuppression against onset of large intestinal cancer characterized inthat after Aspergillus is cultivated and ripened at steamed rice bran, atemperature of ripened product is kept at 44° C. to 46° C., the ripenedproduct (koji) is formed into dried rice bran koji fine powdercontaining dead fungi of Aspergillus while keeping a capability ofcatalysis of enzyme groups produced by Aspergillus and further formedinto fine powder, and the plant protein powder is uniformly mixed withsaid dried rice bran koji fine powder under a state in which there is nodifference in their humidity.
 9. A method for manufacturing a nutritioussupplemental composition for suppression against onset of largeintestinal cancer according to any one of claims 7 and 8 characterizedin that after Aspergillus and lactic acid bacteria are cultivated andripened, the ripened product is formed into dried rice bran kojicontaining dead fungi of Aspergillus and lactic acid bacteria whilekeeping a capability of catalysis of enzyme groups produced byAspergillus.